The Rat ARG1 (Arginase I) ELISA Kit is a cutting-edge tool meticulously designed for the precise and quantitative detection of Arginase I levels in a variety of rat biological samples. Arginase I, a crucial enzyme involved in the urea cycle, plays a fundamental role in the metabolism of arginine to ornithine and urea, impacting various physiological processes including nitrogen balance and the immune response. Accurate measurement of Arginase I levels using this ELISA kit provides researchers with valuable insights into arginine metabolism, cellular proliferation, immune regulation, and tissue repair pathways where this enzyme plays a pivotal role. This ELISA kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results vital for investigating the functions and pathways associated with Arginase I. Manufactured under stringent quality control measures, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for researchers aiming to expand their understanding of arginine metabolism and its implications in health and disease. Trust the Rat ARG1 ELISA Kit to enhance your research capabilities and delve deeper into the complexities of metabolic pathways influenced by Arginase I.
Product Name:
Rat ARG1 (Arginase I) ELISA Kit
SKU:
AEES00316
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
