The Rat BNP (Brain Natriuretic Peptide) ELISA Kit is an advanced assay meticulously developed for the precise quantitative determination of Brain Natriuretic Peptide levels in diverse biological samples obtained from rat subjects. Brain Natriuretic Peptide, a crucial marker of cardiac health, serves as a profound indicator of cardiac stress and heart failure. As a multifaceted peptide hormone, BNP plays a pivotal role in regulating blood pressure, fluid balance, and cardiovascular response, making it a vital target for cardiovascular research, diagnostic evaluations, and therapeutic assessments. This state-of-the-art ELISA kit from Assay Genie boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance, facilitating precise measurements of BNP levels with ease and reliability. Researchers can rely on this ELISA kit to unravel the intricate dynamics of Brain Natriuretic Peptide in cardiovascular pathophysiology and therapeutic interventions, making it an indispensable tool for comprehensive cardiovascular studies.
Product Name:
Rat BNP (Brain Natriuretic Peptide) ELISA Kit
SKU:
AEES00332
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
