The Rat Complement Component 5a (C5a) ELISA Kit is a specialized assay developed to accurately quantify levels of C5a within rat biological samples. Complement Component 5a, a potent pro-inflammatory peptide, plays a crucial role in the activation of the complement system, regulation of immune responses, and modulation of inflammatory processes. This ELISA kit provides researchers with a precise tool to investigate the levels and functions of C5a in various physiological and pathological conditions. Accurate measurement of C5a levels is essential for understanding its involvement in immune system activation, complement-mediated pathways, and inflammatory responses. The Rat C5a ELISA Kit enables researchers to explore the complex mechanisms by which C5a influences immune cell recruitment, cytokine production, and inflammatory cascades. By quantifying C5a in biological samples with high sensitivity and specificity, this kit empowers researchers to uncover valuable insights into the role of C5a in inflammation, infection, and autoimmune disorders. The exceptional sensitivity and specificity of the Rat C5a ELISA Kit ensures accurate and reproducible results, allowing researchers to conduct detailed investigations into the functions and regulatory pathways of C5a. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an ideal choice for research studies focusing on immune modulation, complement system activation, and inflammatory signaling pathways.
Product Name:
Rat C5a (Complement Component 5a) ELISA Kit
SKU:
AEES00527
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
