The Rat Collagen Type I (COL1) ELISA Kit is a specialized assay designed to quantitatively detect Collagen Type I levels in rat biological samples. Collagen Type I is a fundamental component of the extracellular matrix essential for providing structural support and strength to various tissues, including bone, skin, tendon, and ligaments. This ELISA kit serves as a valuable tool for researchers investigating collagen-related processes, tissue remodeling, wound healing, and skeletal biology in rat models. Accurate measurement of Collagen Type I levels is crucial for gaining insights into tissue integrity, regeneration, and the pathophysiology of conditions related to collagen dysregulation. With the Rat COL1 ELISA Kit, researchers can explore the dynamic roles of Collagen Type I in tissue homeostasis and repair, offering a deeper understanding of collagen biology and its implications in various physiological and pathological conditions. Leverage the exceptional sensitivity and specificity of the Rat COL1 ELISA Kit to ensure precise and reproducible results, facilitating in-depth investigations into the intricate functions of Collagen Type I in tissue development, maintenance, and regeneration. Trust in the robust performance of this kit to advance your research in areas concerning extracellular matrix dynamics and tissue physiology.
Product Name:
Rat COL1 (Collagen Type 1) ELISA Kit
SKU:
AEES00524
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
