The Rat Calcitonin (CT) ELISA Kit is a cutting-edge assay meticulously designed for the precise and quantitative measurement of Calcitonin levels in various rat biological samples. Calcitonin, a vital hormone produced by the thyroid, is integral in regulating calcium and phosphorus metabolism, making it essential for bone health and calcium balance in the body. Given its role in modulating calcium levels, Calcitonin is crucial for maintaining bone mineral density and homeostasis. This ELISA kit offers researchers a reliable tool to explore the dynamic changes in Calcitonin levels, shedding light on its involvement in skeletal health, calcium regulation, and potential therapeutic applications. With exceptional sensitivity and specificity, this assay ensures accurate and reproducible results, making it an invaluable asset for investigations into Calcitonin biology in physiological and pathological conditions. Manufactured under stringent quality control standards, this kit guarantees robust performance, providing researchers with a user-friendly and efficient solution for scientific exploration and discovery.
Product Name:
Rat CT (Calcitonin) ELISA Kit
SKU:
AEES00307
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
