The Rat DBP (Vitamin D-binding Protein) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of Vitamin D-binding protein levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers high specificity and reproducibility, providing researchers with consistent and trustworthy results for a variety of research applications.Vitamin D-binding protein, also known as GC protein, plays a crucial role in the transport of Vitamin D metabolites in the circulation and is involved in various physiological processes such as bone health, immune function, and inflammation. Dysregulation of Vitamin D-binding protein levels has been implicated in various diseases including osteoporosis, chronic kidney disease, and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat DBP (Vitamin D-binding Protein) CLIA Kit is a valuable tool for researchers looking to investigate the role of Vitamin D-binding protein in health and disease in rat models, providing accurate and precise measurements for their experiments.
Product Name:
Rat DBP (Vitamin D Binding Protein) CLIA Kit
SKU:
RTES00594
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
