The Rat ET-1 (Endothelin 1) ELISA Kit is a highly specialized assay designed for the quantitative detection of Endothelin 1 (ET-1) levels in rat samples. ET-1 is a potent vasoconstrictor peptide produced in endothelial cells and has essential physiological roles in regulating vascular tone, blood pressure, and kidney function. This ELISA kit provides researchers with a precise method to quantify ET-1 concentrations, enabling investigations into cardiovascular diseases, hypertension, and endothelial dysfunction. Accurate measurement of ET-1 levels using this ELISA kit is crucial for understanding its intricate involvement in vascular physiology, cardiovascular disorders, and renal function. The quantitative data obtained from this assay can shed light on the mechanisms underlying vascular pathophysiology, offering valuable insights into the role of ET-1 in health and disease. Researchers studying cardiovascular conditions can benefit from the detailed analysis of ET-1 levels provided by this kit. Assay Genie's Rat ET-1 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. With stringent quality control measures in place, this kit ensures robust performance and ease of use, making it a reliable choice for cardiovascular research and investigations into the regulatory actions of ET-1 in vascular and renal systems.
Product Name:
Rat ET-1 (Endothelin 1) ELISA Kit
SKU:
AEES00512
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
