The Mouse ET-1 (Endothelin 1) ELISA Kit is specifically designed for the quantitative detection of Endothelin 1 levels in various biological samples collected from mouse models. Endothelin 1 is a crucial vasoconstrictor peptide involved in regulating blood vessel function, blood pressure, and vascular tone. Dysregulation of Endothelin 1 has been implicated in various cardiovascular diseases and conditions, making accurate measurement of its levels vital for understanding vascular pathophysiology and disease mechanisms in mouse research models. Assay Genie's Mouse ET-1 ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance, providing researchers with a reliable tool for the accurate quantification of Endothelin 1 levels in mice. Trust Assay Genie's Mouse ET-1 ELISA Kit for dependable and insightful analysis of this critical vascular peptide in your research studies.
Product Name:
Mouse ET-1 (Endothelin 1) ELISA Kit
SKU:
AEES00195
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
