The Rat FSH (Follicle Stimulating Hormone) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of FSH levels in rat biological samples. Follicle-stimulating hormone (FSH) is a crucial glycoprotein hormone produced by the anterior pituitary gland and plays a pivotal role in the regulation of reproductive processes, specifically in the maintenance of fertility and gametogenesis. Accurate detection and quantification of FSH levels are essential for studying reproductive physiology, fertility issues, and endocrine system disorders. Monitoring changes in FSH concentrations can provide valuable insights into ovarian function and reproductive health. The Rat FSH ELISA Kit enables researchers to investigate the physiological role of FSH and its impact on fertility and reproductive health in rat models. Assay Genie's Rat FSH ELISA Kit offers high sensitivity and specificity, ensuring precise and reproducible results. Manufactured under rigorous quality control standards, this kit guarantees optimal performance and reliability, making it an ideal choice for researchers and scientists involved in reproductive biology and endocrinology research.
Product Name:
Rat FSH (Follicle Stimulating Hormone) ELISA Kit
SKU:
AEES00402
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
