The Rat GS (Glutamine synthetase) ELISA Kit is designed for the quantitative detection of Rat GS (Glutamine synthetase) levels in various biological samples. Glutamine Synthetase (GS/GLUL) is an ATP-dependent enzyme that catalyzes the condensation of glutamate and ammonia to form glutamine, the most abundant amino acid in circulation. It is a critical enzyme for nitrogen homeostasis, providing glutamine for nucleotide biosynthesis, gluconeogenesis, and acid-base balance regulation. In the brain, GS is expressed exclusively in astrocytes and plays an essential role in the glutamate-glutamine cycle, removing excitatory glutamate from synapses. Dysregulation of GS activity is linked to hepatic encephalopathy, neurological disorders, and certain metabolic cancers. Assay Genie's Rat GS (Glutamine synthetase) ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 9.38 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Rat GS (Glutamine synthetase) ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Rat GS (Glutamine synthetase) ELISA Kit
SKU:
AEES00752
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
4 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
