The Rat IFN Beta (Interferon Beta) CLIA Kit is specifically designed for the quantitative detection of IFN Beta levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.IFN Beta is a key cytokine involved in the immune response, playing a crucial role in antiviral defense and modulating inflammatory processes. Dysregulation of IFN Beta levels has been implicated in various autoimmune diseases, making it an important biomarker for studying these conditions and developing targeted therapies. Overall, the Rat IFN Beta CLIA Kit is a valuable tool for researchers looking to study the role of IFN Beta in immune response and inflammatory diseases in rat models. Its high performance and ease of use make it an ideal choice for laboratories conducting cutting-edge research in this field.
Product Name:
Rat IFN- beta (Interferon Beta) CLIA Kit
SKU:
RTES00328
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
