The Rat IL-1α ELISA Kit is a specialized assay designed for the precise quantification of Interleukin-1 alpha (IL-1α) levels in various rat biological samples. IL-1α is a crucial pro-inflammatory cytokine that plays a pivotal role in a wide range of cellular activities, including immune responses, cell proliferation, differentiation, and apoptosis. Its involvement in inflammatory processes and immune regulation makes accurate measurement of IL-1α essential for understanding its role in inflammatory diseases, autoimmune conditions, infections, and other immune-related disorders in rats. The IL-1α ELISA Kit offered by Assay Genie features exceptional sensitivity and specificity, providing reliable and reproducible results for researchers. This kit is manufactured under rigorous quality control standards to ensure robust performance and ease of use, making it a valuable tool for research applications focused on investigating the functions and implications of IL-1α in biological systems.
Product Name:
Rat IL-1alpha ELISA Kit
SKU:
AEES00293
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
