The Rat IL-12 ELISA Kit is a cutting-edge assay meticulously crafted for the quantitative determination of Interleukin-12 (IL-12) levels in diverse rat biological samples. IL-12 is a pivotal cytokine with essential immunological functions, as it orchestrates T cell differentiation, promotes interferon-gamma production, and stimulates the immune response against pathogens. By measuring IL-12 levels accurately, researchers gain insights into immune regulation, adaptive immunity, and inflammatory processes, which are crucial for investigating various diseases and immune-related conditions. Our ELISA kit ensures exceptional sensitivity and specificity, guaranteeing precise and reproducible results vital for advancing your scientific investigations. Manufactured under strict quality control measures, this kit delivers reliable performance and user-friendly protocols, making it a preferred tool for researchers seeking to deepen their understanding of the immunological landscape. Trust the Rat IL-12 ELISA Kit to empower your research endeavors and unlock the mysteries of immune modulation and inflammatory responses.
Product Name:
Rat IL-12 ELISA Kit
SKU:
AEES00314
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
