The Rat LDHA (Lactate Dehydrogenase A) ELISA Kit is specifically designed for the accurate measurement of LDHA levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.LDHA is a key enzyme involved in the conversion of lactate to pyruvate, playing a crucial role in energy metabolism and cellular respiration. Dysregulation of LDHA has been implicated in various diseases such as cancer, cardiovascular disorders, and metabolic diseases, making it a valuable biomarker for studying these conditions and evaluating potential therapeutic interventions. With its user-friendly protocol and reliable performance, the Rat LDHA ELISA Kit is an essential tool for researchers studying LDHA function and its implications in disease pathology. Get accurate and reproducible results with this comprehensive and versatile assay kit.
Product Name:
Rat LDHA (Lactate Dehydrogenase A) ELISA Kit
SKU:
RTES01158
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
