The Human LDHA (Lactate Dehydrogenase A) ELISA Kit is a powerful tool for the precise measurement of LDHA levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for a variety of research investigations.LDHA is an enzyme that plays a key role in cellular metabolism, specifically in the conversion of lactate to pyruvate. Dysregulation of LDHA has been linked to various diseases, including cancer and metabolic disorders, highlighting its importance as a potential biomarker for disease diagnosis and treatment monitoring. By utilizing the Human LDHA ELISA Kit, researchers can gain valuable insights into the role of LDHA in disease pathogenesis and progression, ultimately facilitating the development of novel therapeutic strategies. Trust in the precision and reliability of this kit to advance your research endeavors and uncover new discoveries in the field of biomedical science.
Product Name:
Human LDHA (Lactate Dehydrogenase A) ELISA Kit
SKU:
HUES01740
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
