The Rat Microalbuminuria (MAU) ELISA Kit offers a precise and reliable method for quantitatively detecting microalbuminuria levels in rat biological samples. Microalbuminuria, the presence of small amounts of albumin in urine, serves as an essential marker for early kidney dysfunction, particularly in cardiovascular and renal diseases. Accurate measurement of microalbuminuria plays a critical role in monitoring and diagnosing renal health, providing valuable insights into the progression of kidney disease and cardiovascular complications in rats. With a focus on sensitivity and specificity, Assay Genie's MAU ELISA Kit ensures accurate and reproducible results, making it an excellent tool for researchers investigating renal function, cardiovascular health, and related pathologies in rat models. The kit is manufactured under stringent quality control standards to guarantee robust performance and user-friendly protocols, enhancing its utility for a wide range of research applications.
Product Name:
Rat MAU (Microalbuminuria) ELISA Kit
SKU:
AEES00300
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 μg/mL
Detection range:
1.56-100 μg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
