The Rat MTL (Motilin) ELISA Kit is a specialized assay designed for the quantitative measurement of Motilin levels in various rat biological samples. Motilin, a gastrointestinal hormone produced in the small intestine, plays a crucial role in regulating gastrointestinal motility by stimulating smooth muscle contraction in the digestive tract. This hormone helps coordinate the movement of food and waste through the digestive system, contributing to the digestive process and overall gut health. This ELISA kit enables researchers to accurately quantify Motilin levels in rat samples, providing valuable insights into gastrointestinal function, motility disorders, and related physiological processes. With exceptional sensitivity and specificity, the Rat MTL ELISA Kit offers reliable and reproducible results, allowing for in-depth investigations into motilin's biological effects. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an excellent tool for research focused on gastrointestinal physiology and digestive health in rat models.
Product Name:
Rat MTL (Motilin) ELISA Kit
SKU:
AEES00487
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
