The Human MTL (Motilin) ELISA Kit is specially designed to detect motilin levels in human biological samples such as serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.Motilin, a peptide hormone, plays a vital role in gastrointestinal motility by stimulating the contraction of smooth muscles in the digestive system. Dysregulation of motilin levels has been linked to disorders such as gastric stasis and other gastrointestinal issues, making it a valuable biomarker for studying and potentially treating these conditions. By utilizing the Human MTL ELISA Kit, researchers can gain valuable insights into the role of motilin in gastrointestinal health and diseases, paving the way for advancements in diagnosis and treatment options. Trust in this kit for precise measurements and impactful discoveries in the field of gastrointestinal research.
Product Name:
Human MTL (Motilin) ELISA Kit
SKU:
HUES02973
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
