Rat NEFL (Neurofilament, Light Polypeptide) ELISA Kit
The Rat NEFL (Neurofilament Light Polypeptide) ELISA Kit is specifically designed for the quantitative measurement of NEFL levels in rat serum, plasma, and tissue homogenates. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reliable results for various research applications.NEFL is a key component of the neurofilament protein complex found in neurons, essential for maintaining neuronal structure and function. Abnormal levels of NEFL have been linked to neurodegenerative diseases such as Alzheimer's and multiple sclerosis, highlighting its significance as a potential biomarker for studying these conditions and developing therapeutic strategies. Overall, the Rat NEFL ELISA Kit offers a valuable tool for researchers investigating neurodegenerative disorders and seeking to understand the role of NEFL in disease progression. Its precision and versatility make it an indispensable resource for advancing scientific knowledge in the field.
Product Name:
Rat NEFL (Neurofilament, Light Polypeptide) ELISA Kit
SKU:
RTES01149
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
