Rat OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
The Rat OxLDL (Oxidized Low-Density Lipoprotein) ELISA Kit is expertly crafted to facilitate the precise measurement of Oxidized LDL levels in diverse rat biological specimens. OxLDL, a modified version of LDL cholesterol, is acclaimed for its pivotal role in atherogenesis and cardiovascular disorders. As an influential pro-atherogenic factor, OxLDL is involved in a myriad of pathophysiological processes, including endothelial dysfunction, foam cell formation, and inflammation within the arterial walls. Accurate quantification of Oxidized LDL using this ELISA kit offers invaluable insights into the mechanisms underlying atherosclerosis and related cardiovascular complications. Understanding the impact of OxLDL on lipid metabolism, vascular health, and atherosclerotic plaque development is crucial for unveiling novel therapeutic strategies and diagnostic approaches in the realm of cardiovascular research. The Rat OxLDL (Oxidized Low-Density Lipoprotein) ELISA Kit from Assay Genie guarantees exceptional sensitivity and specificity, ensuring precise and reproducible outcomes. With a commitment to stringent quality control standards, this kit delivers robust performance and user-friendly operation, making it a prime choice for cutting-edge research in cardiovascular health and disease.
Product Name:
Rat OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
SKU:
AEES00502
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.