The Rat PCT (Procalcitonin) ELISA Kit is specifically designed for the precise measurement of procalcitonin levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it perfect for various research applications.Procalcitonin is an important biomarker linked to systemic inflammation and bacterial infections. Its levels rise significantly in response to bacterial infections, making it a valuable tool for diagnosing sepsis or monitoring antibiotic therapy. By providing reliable data on procalcitonin levels, this ELISA kit can aid in the treatment and management of infectious diseases in rats.Overall, the Rat PCT (Procalcitonin) ELISA Kit is a valuable tool for researchers studying inflammatory conditions in rats, offering a reliable method for quantifying procalcitonin levels and advancing our understanding of infectious diseases.
Product Name:
Rat PCT (Procalcitonin) ELISA Kit
SKU:
RTES01028
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.