The Rat PGF (Placental Growth Factor) CLIA Kit is a cutting-edge assay kit designed for the accurate measurement of PGF levels in rat samples, including serum, plasma, and tissue homogenates. With high sensitivity and specificity, this kit ensures precise and reliable results for a variety of research applications.PGF, a member of the VEGF family, is a key player in angiogenesis and vasculogenesis processes, crucial for the development of blood vessels and tissue regeneration.
Dysregulation of PGF has been implicated in various diseases such as cancer, cardiovascular disorders, and placental insufficiency. Therefore, this kit serves as a valuable tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat PGF CLIA Kit provides researchers with a powerful tool for investigating the role of PGF in physiological and pathological processes, advancing our understanding of angiogenesis and vascular disorders.
Product Name:
Rat PGF (Placental Growth Factor) CLIA Kit
SKU:
RTES00440
Target:
Rat PGF (Placental Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PGF. You can calculate the concentration of Rat PGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
85-99
102-114
Average (%)
98
91
109
1:4
Range (%)
94-105
101-114
85-95
Average (%)
99
106
90
1:8
Range (%)
101-120
91-107
103-117
Average (%)
110
97
108
1:16
Range (%)
92-107
89-102
98-114
Average (%)
99
94
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
104
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
97-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.38
23.95
97.83
8.91
22.41
100.36
Standard deviation
0.97
2.16
7.87
0.87
2.2
8.68
C V (%)
10.34
9.02
8.04
9.76
9.82
8.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PGF in samples. No significant cross-reactivity or interference between Rat PGF and analogues was observed.
