The Rat SHBG (Sex Hormone Binding Globulin) ELISA Kit is specifically designed for the quantitative measurement of SHBG levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.SHBG is a critical protein that binds to sex hormones such as testosterone and estrogen, regulating their bioavailability and activity. Changes in SHBG levels have been associated with various hormonal disorders, metabolic diseases, and reproductive health conditions. By using this ELISA kit, researchers can study the role of SHBG in these conditions and explore potential therapeutic interventions.Overall, the Rat SHBG ELISA Kit provides a valuable tool for investigating the role of SHBG in physiological processes and pathological conditions, offering researchers a deeper understanding of hormonal regulation and potential treatment strategies.
Product Name:
Rat SHBG (Sex Hormone-Binding Globulin) ELISA Kit
SKU:
RTES00736
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
