Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don't inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.
Description
Background Information
| Information | Description |
| Product Name: | Recombinant Human ACE2 Protein (Avi-His Tag) (Activity Tested) |
| Size: | 20µg |
| SKU: | RPES20029 |
| Activity: | Immobilized 2019-nCoV S1 Protein-Fc(Cat#RPES0016) at 2ug/ml (100 uL/well) can bind Biotinylated Human ACE-2-Avi-His (Cat#RPES0029). The ED50 of Biotinylated Human ACE-2-Avi-His (Cat#RPES0029) is 0.17 ug/ml. |
| Synonym: | Angiotensin-Converting Enzyme 2; ACE-Related Carboxypeptidase; Angiotensin-Converting Enzyme Homolog; ACEH; Metalloprotease MPROT15; ACE2 |
| Protein Construction: | Biotinylated Recombinant Human Angiotensin-Converting Enzyme 2 is produced by our Mammalian expression system and the target gene encoding Gln18-Ser740 is expressed with a Avi, 6His tag at the C-terminus. |
| Sequence: | Gln18-Ser740 |
| Fusion tag: | C-Avi-6His |
| Accession: | Q9BYF1 |
| Species: | Human |
| Expressed Host: | Human Cells |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin: | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20? for 3 months. |
| Mol Mass: | 86.2 kDa |
| AP Mol Mass: | 110-140 kDa |
| Formulation: | Supplied as a 0.2 uM filtered solution of 20mM Tris-HCl, 300mM NaCl, 1mM ZnCl2, 10% Glycerol, pH 7.4. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
