TNFRSF21 (death receptor-6; DR6) is an orphan TNF receptor superfamily member and belongs to a subgroup of receptors called death receptors. This type I transmembrane receptor possesses four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is an extensively posttranslationally modified transmembrane protein and that N- and O-glycosylations of amino acids in its extracellular part. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain; and expression of DR6 in mammalian cells induces activation of both NF-kappaB and JNK and cell apoptosis. DR6 knockout mice have enhanced CD4+ T cell proliferation and Th2 cytokine production; suggested that DR6 serves as an important regulatory molecule in T-helper cell activation; and is involved in inflammation and immune regulation. DR6 is expressed ubiquitously with high expression in lymphoid organs; heart; brain and pancreas. Some tumor cells overexpress DR6; typically in conjunction with elevated anti-apoptosis molecules. DR6 may also be involved in tumor cell survival and immune evasion; which is subject to future investigations.
Product Name:
Human DR6/TNFRSF21 Recombinant Protein (RPES0946)
Product Code:
RPES0946
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Tumor Necrosis Factor Receptor Superfamily Member 21, Death Receptor 6, CD358, TNFRSF21, DR6
Accession:
NP_055267.1
Sequence:
Met 1-Leu 350
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA.2. Immobilized recombinant human DR6-Fc at 10 µg/mL (100 ?l/well) can bind biotinylated human APP-Fc with a linear range of 0.03-0.25 µg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Leu 350) of human DR6 (NP_055267.1) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
60.3 kDa
AP Mol Mass:
95-100 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
