IL-15 (interleukin 15) is a cytokine that controls T cell and natural killer cell activation and proliferation. IL-15 binds to the alpha subunit of the IL15 receptor (IL-15RA) with high affinity. It binds to the IL-2 receptor's beta and gamma chains, but not the alpha subunit. IL-15 is structurally and functionally related to IL-2. Both cytokines have overlapping subunits of receptors, allowing them to battle for and limit one other's activity. The number of CD8+ memory T cells is regulated by a balance between IL-15 and IL-2. Despite the fact that they have many overlapping immunological functions, IL-2 and IL-15 are actually quite distinct players in the immune system. IL-15 is produced by a wide range of cells, including monocytes, macrophages, and DCs. Mature Human IL-15 is a protein that has 70% amino acid sequence similarity with Mouse and Rat IL-15. Human Interleukin5/IL5 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin5/IL5 Recombinant Protein (RPES1731)
Product Code:
RPES1731
Size:
20 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
IL-15,Interleukin 15,IL15
Accession:
P40933
Sequence:
Asn49-Ser162
Activity:
Measured in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells. The ED50 for this effect is 40-200pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-15 is produced by our E.coli expression system and the target gene encoding Asn49-Ser162 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
12.5 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
