Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region; a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates; such that mature human Noggin shares 99%; 99%; 98%; 97% and 89% aa sequence identity with mouse; rat bovine; equine and chicken Noggin; respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung; skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions; addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state; or alternatively; to differentiate into dopaminergic neurons.
Product Name:
Human Noggin/NOG Recombinant Protein (RPES0741)
Product Code:
RPES0741
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Noggin,SYM1,SYNS1
Accession:
Q13253
Sequence:
Gln28-Cys232
Fusion tag:
C-Fc
Activity:
Immobilized Human BMP-2(Cat: PKSR030454) at 2µg/ml(100 ?l/well) can bind Human Noggin-Fc. The ED50 of Human Noggin-Fc is 0.045µg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encodingGln28-Cys232 is expressed with a Fc tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
50.2 kDa
AP Mol Mass:
60 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS;pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
