The EF-hand protein S100A1 is a Ca2+ binding enzyme that belongs to the S100 protein family. S100 proteins are dimers in the cytoplasm and/or nucleus of a variety of cells, many of which are involved in the regulation of several cellular activities such as cell-cycle progression and cell differentiation. Furthermore, it has been demonstrated to participate in the actions of stimulation of Ca2+-induced Ca2+ release, microtubule assembly inhibition, and PKC-mediated phosphorylation inhibition. Phosphoglucomutase is a protein target that is antagonistically regulated by S100A1, and lately, it has been discovered to be a strong molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Reduced expression or mutations of S100A1 gene have been linked with cardiomyopathies. Human S100A1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human S100A1 Recombinant Protein (RPES0819)
Product Code:
RPES0819
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
S100,S100-alpha,S100A
Accession:
NP_006262.1
Sequence:
Met 1-Ser 94
Fusion tag:
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized recombinant human Fc-S100B at 10 µg/mL (100 ?l/well) can bind biotinylated human S100A1 with a linear range of 15.6-250 ng/mL.3. Measured by its ability to bind human His-S100B in functional ELISA.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human S100A1 (NP_006262.1) (Met 1-Ser 94) was expressed.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
10.5 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
