LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells. It also acts as as a new marker of T cell induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signalling, leading to increased antigen-specific T-cell responses in vivo.Immune CheckpointImmune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Product Name:
Rat LAG3/CD223 Recombinant Protein (RPES4468)
Product Code:
RPES4468
Size:
100 µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
LAG3
Accession:
Q5BK54
Sequence:
Met1-Leu442
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat LAG3 (Q5BK54)(Met1-Leu442) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
47.1 kDa
AP Mol Mass:
55 kDa
Formulation:
Lyophilized from sterile 20 mM NaAc, 500 mM NaCl, 10 % Glycerol, 0.02 % tween 20, pH 5.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
