The Rig-I/DDX58 Monoclonal Antibody (CAB22478) is a high-quality antibody developed for reliable detection and analysis of target proteins. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Rig-I/DDX58 Monoclonal Antibody
SKU:
CAB22478
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC54110
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
Purification Method
Affinity purification
Gene ID
23586
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Rig-I/DDX58 Rabbit mAb (CAB22478) at 1:2000 dilution. THP-1 cells were treated with LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of lysates from RAW264.7 cells, using Rig-I/DDX58 Rabbit mAb (CAB22478) at 1:2000 dilution. Raw264. 7 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
