SARS-CoV-2 3C-like Proteinase Recombinant Protein (His Tag) (RPES0001)

SKU:
RPES0001
€300

Description

SARS-CoV 3CL (3C-like Proteinase)

The viral main proteinase (M pro , also called 3CL pro ), which controls the activities of the coronavirus replication complex. It functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents.

Key Information Description

Product Name:

Recombinant SARS-CoV-2 3C-like Proteinase Protein (His Tag)

Size:

10µg

SKU:

RPES0001

Activity:
Synonym:
M Proteinase; 3CL Proteinase
Protein Construction:
SARS-Cov-2 3C-like Proteinase is produced by our E.coli expression system and the target gene encoding Ser1-Gln306 is expressed with a 6His tag at the N-terminus.
Sequence:
Ser1-Gln306
Fusion tag:
N-6His
Accession:
YP_009725301.1
Species:
virus
Expressed Host:
E.coli
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Endotoxin:
< 1.0 EU per µg as determined by the LAL method.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Mol Mass:

36.1 kDa

AP Mol Mass:
35 kDa
Formulation:
Supplied as a 0.2 uM filtered solution of PBS, 1mM EDTA, 10% Glycerol, pH 7.4.
Reconstitution:
Please refer to the printed manual for detailed information.

Uniprot:

SARS-CoV 3CL (3C-like Proteinase) - Protein Information

Uniprot Protein Function:

  • Cysteine-type endopeptidase activity
  • Single-stranded RNA binding
  • Thiol-dependent ubiquitinyl hydrolase activity
  • Transferase activity
  • Zinc ion binding

Uniprot Protein Details:

Biological Process:

  • Induction by virus of catabolism of host mRNA
  • Induction by virus of host autophagy
  • Modulation by virus of host protein ubiquitination
  • Suppression by virus of host type I interferon-mediated signaling pathway 
  • Viral genome replication 
  • Viral protein processing

Organism:

Severe acute respiratory syndrome coronavirus (SARS-CoV).

Ligand:

Metal-binding, Zinc

Alternative Names:

Growth factor-like peptide, Leader protein, Non-structural protein 10, Non-structural protein 2, Non-structural protein 3, Non-structural protein 4, Non-structural protein 5, Non-structural protein 6, Non-structural protein 7, Non-structural protein 8, Non-structural protein 9, Papain-like proteinase, p65 homolog.

Virus Host:

Homo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]

Subcellualar Location:

  • Host perinuclear region  
  • Host membrane ; Multi-pass membrane protein  
  • Membrane

Uniprot Code:

NCBI GenInfo Identifier:

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Additional Information

Product type:
Recombinant Protein
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