The TNFRSF10A Antibody (CAB12540) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. RRID AB_2759380 Gene ID 8797 Swiss Prot Synonym DR4; APO2; CD261; TRAILR1; TRAILR-1; TNFRSF10A
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TNFRSF10A Antibody
SKU:
CAB12540
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:100
IF-P
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DR4, APO2, CD261, TRAILR1, TRAILR-1, TNFRSF10A
Positive Sample:
K-562, SW480, MCF7, HeLa, DU145, Mouse lung, Rat lung
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
50kDa
Observed MW:
40-55kDa
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. RRID AB_2759380 Gene ID 8797 Swiss Prot Synonym DR4; APO2; CD261; TRAILR1; TRAILR-1; TNFRSF10A
Purification Method:
Affinity purification
Gene ID:
8797
RRID:
AB_2759380
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TNFRSF10A Rabbit pAb (CAB12540) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of THP-1 cells using TNFRSF10A Rabbit pAb (CAB12540) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
