Topoisomerase II alpha Rabbit Monoclonal Antibody (CAB4389)

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CAB4389
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Description

Technical Manual

Topoisomerase II alpha Rabbit Monoclonal Antibody

Product Name:Topoisomerase II alpha Rabbit Monoclonal Antibody
Product SKU:CAB4389
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Immunogen:A synthesized peptide derived from human Topoisomerase II alpha
Sequence:

Email for sequence

Gene ID:7153
Swiss Prot:P11388
Synonyms:TOP2; TP2A
Calculated MW:174kDa
Observed MW:190KDa
Reactivity:Human, Mouse
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting 
Positive Samples:HeLa, Mouse testis
Cellular Location:

Product Images

Topoisomerase II alpha Rabbit Monoclonal Antibody (CAB4389)

Western blot - Topoisomerase II alpha Rabbit mAb (CAB4389)

Uniprot Information

UniProt Protein Function:Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation.
NCBI Summary:This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
UniProt Code:P11388
NCBI GenInfo Identifier:13959709
NCBI Gene ID:7153
NCBI Accession:P11388.3
UniProt Secondary Accession:P11388,Q71UN1, Q71UQ5, Q9HB24, Q9HB25, Q9HB26, Q9UP44 Q9UQP9, B2RTS1,
UniProt Related Accession:P11388
Molecular Weight:182,681 Da
NCBI Full Name:DNA topoisomerase 2-alpha
NCBI Synonym Full Names:topoisomerase (DNA) II alpha
NCBI Official Symbol:TOP2A 
NCBI Official Synonym Symbols:TOP2; TP2A 
NCBI Protein Information:DNA topoisomerase 2-alpha
UniProt Protein Name:DNA topoisomerase 2-alpha
UniProt Synonym Protein Names:DNA topoisomerase II, alpha isozyme
Protein Family:DNA topoisomerase
UniProt Gene Name:TOP2A 
UniProt Entry Name:TOP2A_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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