Description
| Product Name: | Total Cholesterol and Cholesteryl Ester Assay Kit - Fluorometric |
| Product Code: | MAES0007 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue, cells |
| Assay Time: | 20 min |
| Instrument: | Fluorescence Microplate Reader |
| Sensitivity: | 0.12 µmol/L |
| Detection Range: | 0.12-30 µmol/L |
| Recovery Rate: | 96 |
| Inter CV: | 7.3 |
| Inter CV: | 1.7 |
Cholesterol is a kind of sterol and lipid in cell membrane. Most of cholesterol in blood exists in the form of cholesterol ester. Lecithin-cholesterol acyltransferase in human plasma is an enzyme that catalyzes the formation of cholesterol ester. Cholesterol synthesized or deposited in peripheral cells returns to the liver through the reverse cholesterol transport system for reusing or regaining bile acids.
Total cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce â–³4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Lipids metabolism |
