Description
Uric Acid Assay Kit (Colorimetric) (BA0049) (BA0049)
The Uric Acid Assay Kit (Colorimetric) (SKU: BA0049) provides a simple, direct and automation-ready method for measuring uric acid directly in serum without any pretreatment. Uric acid is the waste product produced from the degradation of purines and, in healthy humans, is filtered and removed from the blood by the kidneys and excreted into urine. Because a number of kidney diseases affect uric acid levels, its determination is useful in diagnosing and evaluating kidney disease; abnormally high levels can crystallise in body joints to cause gout, and raised levels are also associated with uraemia, leukaemia and pneumonia. The improved method utilises 2,4,6-tripyridyl-s-triazine, which forms a blue-coloured complex specifically with iron in the presence of uric acid. The intensity of the colour, measured at 590nm, is directly proportional to the uric acid concentration. Only 5 µL of sample is required and the optimised formulation substantially reduces interference by substances in raw samples.
| Product Name: | Uric Acid Assay Kit (Colorimetric) (BA0049) |
| SKU: | BA0049 |
| Detection Method: | Colorimetric |
| Detection Range: | 0.22 mg/dL (13 µM) to 30 mg/dL (1785 µM) uric acid in 96-well plate assay |
| Sample Type: | Serum, plasma, urine and other biological samples |
| Species Reactivity: | All |
| Assay Time: | 30 minutes |
| Kit Size: | 250 Assays |
| Equipment Required: | Microplate reader |
| Storage: | The kit is shipped at room temperature. Store reagents at 4°C, and standard and blank control at -20°C. |
| Shelf Life: | 12 months after receipt |
| Shipping: | Room Temperature |
This kit measures uric acid directly in serum without pretreatment. The improved method utilises 2,4,6-tripyridyl-s-triazine, which forms a blue-coloured complex specifically with iron in the presence of uric acid. The intensity of the colour, measured at 590nm, is directly proportional to the uric acid concentration in the serum.
- Sensitive and accurate: use 5 µL samples; linear detection range 0.22 mg/dL (13 µM) to 30 mg/dL (1785 µM) uric acid in 96-well plate assay
- Simple and high-throughput: addition of a single working reagent and incubation for 30 minutes, readily automated for thousands of samples per day
- Improved reagent stability and versatility in cuvette or 96-well plate format
- Low interference in biological samples: no pretreatments required, assays performed directly on serum
- Direct assays: uric acid in serum, plasma, urine and other biological samples
- Drug discovery/pharmacology: effects of drugs on uric acid metabolism
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Shake Reagent C before use and prepare Working Reagent by mixing 10 volumes Reagent A, 1 volume Reagent B and 1 volume Reagent C; equilibrate to room temperature. Metal chelators (e.g. EDTA) should be avoided. |
| 2 | Set up standards and samples: transfer 5 µL Blank, Standard and samples in duplicate wells of a clear bottom 96-well plate. |
| 3 | Add 200 µL Working Reagent and tap lightly to mix. |
| 4 | Incubate 30 minutes at room temperature and read optical density at 510-630nm (peak absorbance at 590nm). |
Uric acid concentration = [(ODSAMPLE − ODBLANK) / (ODSTANDARD − ODBLANK)] × 10 (mg/dL), where ODBLANK, ODSTANDARD and ODSAMPLE are OD590nm values of Blank, Standard and Sample. A calibration curve is not required, because the provided standard lies within the linear range. Normal serum uric acid values: 1.0 to 7.0 mg/dL. Conversion: 1 mg/dL uric acid equals 59.5 µM, 0.001% or 10 ppm.
| Component | Quantity | Storage |
| Reagent A | 50 mL | 4°C |
| Reagent B | 6 mL | 4°C |
| Reagent C | 6 mL | 4°C |
| Standard (10 mg/dL uric acid) | 1 mL | -20°C |
| Blank Control | 1 mL | -20°C |
