The 2'-O-Methylguanosine(Gm) Monoclonal Antibody (CAB20696) is a high-quality antibody developed for reliable detection and analysis of target proteins. RNA methylation plays a significant regulatory role in various of physiological activities and it has gradually become a hotspot of epigenetics in the past decade. 2'-O-methyladenosine (Am), 2'-O-methylguanosine (Gm),2'-O-methylcytidine (Cm),2'-O-methyluridine (Um),N 6-methyladenosine (m6A), N 1-methylguanosine (m1G),5-methylcytidine (m5C),and 5-methyluridine (m5U) are representative 2'-O-methylation and base-methylation modified epigenetic marks of RNA. 2'-O-methyltransferase is a modified nucleoside that is produced in tRNAs by the action of tRNA guanosine-2'-O-methyltransferase, using S-adenosyl-L-methionine as a substrate. Through its interaction with other modified nucleosides, 2'-O-methylguanosine is thought to stabilize the structure of the tRNA.
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Species independent samples.
Product Name:
2'-O-Methylguanosine(Gm) Monoclonal Antibody
SKU:
CAB20696
Size:
100μL, 20μL
Reactivity:
Species independent
Clone Number:
ARC50772
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to 2'-O-Methylguanosine(Gm).
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
RNA methylation plays a significant regulatory role in various of physiological activities and it has gradually become a hotspot of epigenetics in the past decade. 2'-O-methyladenosine (Am), 2'-O-methylguanosine (Gm),2'-O-methylcytidine (Cm),2'-O-methyluridine (Um),N 6-methyladenosine (m6A), N 1-methylguanosine (m1G),5-methylcytidine (m5C),and 5-methyluridine (m5U) are representative 2'-O-methylation and base-methylation modified epigenetic marks of RNA. 2'-O-methyltransferase is a modified nucleoside that is produced in tRNAs by the action of tRNA guanosine-2'-O-methyltransferase, using S-adenosyl-L-methionine as a substrate. Through its interaction with other modified nucleosides, 2'-O-methylguanosine is thought to stabilize the structure of the tRNA.
Purification Method
Affinity purification
Gene ID
CAS: 2140-71-8
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
The 2'-O-methylguanosine(Gm) Rabbit mAb (CAB20696) are tested in Dot Blot against 2'-O-methylguanosine and unmodified guanosine.2'-O-methylguanosine：Biotin-5'GCATAATGACTACT(Gm)3'unmodified guanosine：Biotin-5'GCATAATGACTACTG3'
