The BCHE Monoclonal Antibody (CAB8861) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. RRID Gene ID 590 Swiss Prot Synonym E1; CHE1; CHE2; BCHED; Butyrylcholinesterase
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
BCHE Monoclonal Antibody
SKU:
CAB8861
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC1328
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. RRID Gene ID 590 Swiss Prot Synonym E1; CHE1; CHE2; BCHED; Butyrylcholinesterase
Purification Method:
Affinity purification
Gene ID:
590
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Butyrylcholinesterase Rabbit mAb (CAB8861) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
