Histone H1.2 Rabbit Monoclonal Antibody (CAB0646)
- SKU:
- CAB0646
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- H1.2
- H1C
- H1F2
- H1s-1
Frequently bought together:
Description
| Product Name: | Histone H1.2 Rabbit Monoclonal Antibody |
| SKU: | CAB0646 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat,Other (Wide Range Predicted) |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H1.2 (P16403). |
| Sequence: | MSET APAA PAAA PPAE KAPV KKKA AKKA GGTP RKAS GPPV SELI TKAV AASK ERSG VSLA ALKK ALAA AGYD VEKN NSRI KLGL KSLV SKGT LVQT KGTG |
| Tested Applications: | WB IF/ICC ELISA |
| Recommended Dilution: | WB,1:1000 -1:5000 IF/ICC,1:50 - 1:500 |
| Synonyms: | H1C; H1.2; H1F2; H1s-1; HIST1H1C; Histone H1.2 |
| Positive Sample: | HeLa,Hep G2,Raji,Mouse thymus,Rat lung |
| Conjugate: | Unconjugated |
| Cellular Localization: | nucleus |
| Calculated MW: | 21kDa |
| Observed MW: | 32kDa |
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
| Purification Method: | Affinity purification |
| Gene ID: | 3006 |
| Clone Number: | ARC1836 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using Histone H1.2 Rabbit mAb (CAB0646) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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