The LSD2/KDM1B/AOF1 Monoclonal Antibody (CAB19820) is a high-quality antibody developed for reliable detection and analysis of target proteins. Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LSD2/KDM1B/AOF1 Monoclonal Antibody
SKU:
CAB19820
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2347
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AOF1, LSD2, C6orf193, LSD2/KDM1B/AOF1
Positive Sample:
HeLa, HCT 116, NIH/3T3, PC-12, C6
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
92 kDa
Observed MW:
92 kDa
Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).
Purification Method
Affinity purification
Gene ID
221656
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LSD2/KDM1B/AOF1 Rabbit mAb (CAB19820) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using LSD2/KDM1B/AOF1 Rabbit mAb (CAB19820) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
