Anti-Mouse/Rat CD90.1 (Thy-1.1) (Clone OX-7) In Vivo Antibody

Product Name:	Anti-Mouse/Rat CD90.1 (Thy-1.1) (Clone OX-7) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0302
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	OX-7
Protein:	CD90.1
Product Type:	Monoclonal Antibody
Synonyms:	CD90, CD90.1, Thy-1, Thy1.1, Thy1.2, Thy-1.2
Isotype:	IgG1
Reactivity:	Mouse, Rat
Applications:	Depletion, FC, IF, IHC, In Vivo
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	<1.0 EU/µg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	Depletion, FC, IF, IHC, In Vivo
Reactivity:	Mouse/Rat

Host Species:	Mouse
Specificity:	Clone OX-7 activity is directed against both rat and murine CD90/Thy-1.
Antigen Distribution:	CD90 is present on a variety of cell types in murine and rat, including lymphatic vessels1, T cells2, neurons3 and fibroblasts4.
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<1.0 EU/µg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD90/Thy-1 (murine CD90.1/Thy-1.1) is a cell surface glycoprotein that was the first protein of the immunoglobulin superfamily to be discovered5. The 25 kDa core protein is N-glycosylated at three sites, leading to isoforms with a range of molecular masses (25-37 kDa). CD90 is composed of a single V-like immunoglobulin domain anchored by a disulfide bond between Cys 28 and Cys 104. Despite its lack of an intracellular domain, CD90 is located in the outer leaflet of lipid rafts at the cell membrane, allowing signaling functions by cis- and trans-interactions with a variety of proteins, including G inhibitory proteins, the Src family kinase (SFK) members src and c-fyn, and tubulin.

CD90 has been found to play a role in numerous cellular activities, such as cell adhesion, apoptosis, metastasis, inflammation, and fibrosis. In mouse strains expressing CD90.1, it is expressed on early-stage hematopoietic cells in bone marrow, thymocytes, and circulating mature T cells. The OX-7 antibody has been reported to induce leukocyte activation, glomerular nephritis, apoptosis in glomerular mesangial cells, and vascular permeability. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

Protein:	CD90.1
Function:	The function of Thy1 has not been fully elucidated but is thought to play roles in regulation of cell adhesion, apoptosis, metastasis, inflammation, and fibrosis
Research Area:	Immunology
Shipping:	2-8°C
NCBI Gene Bank ID:	21838
UniProt:	CD90