The RGMA Monoclonal Antibody (CAB0865) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for RGMA and has been validated for use in Western blot and immunohistochemistry applications.RGMA is involved in regulating the growth of axons in the central nervous system, making it an important target for studies in neuroscience and developmental biology. Its role in guiding axon growth and nerve regeneration makes it of interest in research on neurological disorders, spinal cord injuries, and brain development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
RGMA Monoclonal Antibody
SKU:
CAB0865
Size:
20μL, 100μL
Reactivity:
Rat
Clone Number:
ARC2546
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RGM, RGMA
Positive Sample:
Rat skeletal muscle
Cellular Localization:
Cell Membrane, Gpi-Anchor, Lipid-Anchor.
Calculated MW:
49kDa
Observed MW:
60kDa
This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
56963
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat skeletal muscle using RGMA Rabbit mAb (CAB0865) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
