Phosphorylation Assays
Cell-based assays that measure ERK and NF-kB phosphorylation directly in the culture plate.
A read-out of MAPK pathway activation downstream of growth factors and receptor tyrosine kinases.
A fluorometric read-out of NF-kB pathway activation in inflammation and immune signalling.
For a wider range of targets, see our ELISA kit range.
Phosphorylation is the main switch in cell signalling. Cell-based assays measure the phosphorylated protein in fixed cells in the well, which removes lysate preparation and makes it practical to compare many treatments or time points on one plate.
A western blot resolves proteins by size and is low throughput. A cell-based assay gives a quantitative plate-reader signal across many wells without gels or transfer.
No. These assays are run on cells in the culture plate.
These kits cover ERK and NF-kB. Phospho-specific ELISA kits cover a wider range of targets.