NFkB Phosphorylation Assay Kit (Fluorometric) (BA0136)
NFkB Phosphorylation Assay Kit
Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-alpha. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcriptionAssay Genie's cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.
ApplicationsFor quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.
- Simple and convenient. No cell lysis necessary, cells can be cultured for several days.
NFkB Phosphorylation Assay Kit Information:
|Kit Includes:||10x Wash Buffer: 25 mL, Blocking Buffer: 25 mL, Protein Stain: 6 mL, HRP Substrate: 6 mL, pNF-kappaB-Ab1: 10 uL HRP-Ab2 10 uL|
|Kit Requires:||37% formaldehyde; 3% H2O2 ; black cell culture 96-well plate; plate sealers; deionized or distilled water; pipetting devices; cell culture incubators; centrifuge tubes; fluorescence plate reader capable of reading at ex/em =530/585 nm and at ex/em =360/450 nm|
|Method of Detection:||FL360/450nm, 530/585nm|
|Samples:||Cell, tissue etc|
|Storage:||Store all reagents at -20°C|
|Shelf Life:||6 months|