Labeling Kits
Conjugate your own antibody or protein to a fluorescent dye or biotin. Each kit supplies the reagents needed to label molecules containing primary amine groups.
FITC, AF430, AF488, AF568, AF594, AF647, AF680, AF700, AF750, AFViolet 450 and AFRed 780.
Cyanine3, Cyanine5, Cyanine5.5 and Cyanine7.
Standard, long-arm, water-soluble and chromogenic biotin labeling kits.
Start with the label that fits a free channel on your instrument, or biotin if you plan to detect with streptavidin. Each label is then offered in versions that differ in the filtration tube supplied for purification, for example 3 kDa or 10 kDa cut-off, so choose the one suited to the size of your protein. The cut-off is stated under Applications on each product page.
The dyes react with primary amines, so remove Tris, glycine, sodium azide and carrier proteins such as BSA from the protein before labeling. A BSA removal kit is available in assay reagents.
Exchange into an amine-free buffer and remove carrier protein.
Mix with the reactive dye or biotin and incubate.
Remove unreacted label with the filtration tube supplied.
Antibodies and other proteins that contain primary amine groups.
They differ in the filtration tube supplied for clean-up, to suit proteins of different sizes. Check the Applications line on each product page.
Over-labeling can reduce binding. Follow the recommended protein-to-label ratio and test the conjugate against the unlabeled antibody.
Anything containing primary amines, such as Tris or glycine, as well as sodium azide and carrier proteins.