The GTF2F2 Antibody (CAB5826) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding to the GTF2F2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and gene regulation.GTF2F2 is essential for the initiation of transcription and plays a crucial role in regulating gene expression in various cellular processes. Its involvement in RNA polymerase II-mediated transcription makes it a valuable target for research in areas such as gene regulation, chromatin organization, and transcriptional control mechanisms.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GTF2F2 Antibody
SKU:
CAB5826
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BTF4, RAP30, TF2F2, TFIIF, GTF2F2
Positive Sample:
SW480, 293T, HepG2, HL-60, A-549
Cellular Localization:
Nucleus.
Calculated MW:
28kDa
Observed MW:
29kDa
Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription preinitiation complex.
Purification Method
Affinity purification
Gene ID
2963
RRID
AB_2766578
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GTF2F2 Rabbit pAb (CAB5826) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using GTF2F2 Rabbit pAb (CAB5826). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
