Description
Human Cancer Discovery Array (20 targets) (SARB0028)
The Human Cancer Discovery Array (SARB0028) offered by Assaygenie is a powerful tool for researchers conducting studies on cancer and immune regulation. This array targets 20 key proteins involved in cancer development and immune response, providing valuable insights into the molecular mechanisms underlying these processes.With a focus on immune regulation, this array includes targets such as CD300A, a cell surface molecule known for its role in inhibiting immune responses. By detecting and analyzing the expression levels of these key proteins, researchers can gain a better understanding of the complex interplay between the immune system and cancer cells.
The Human Cancer Discovery Array is suitable for a wide range of applications, including Western blotting and other protein detection techniques. With its high reactivity towards human samples, researchers can trust the accuracy and reliability of their results when using this array.Overall, the Human Cancer Discovery Array (SARB0028) from Assaygenie is an invaluable tool for researchers seeking to unravel the complexities of cancer and immune regulation. By studying the expression patterns of key proteins involved in these processes, researchers can move closer to developing targeted therapies for cancer and other immune-related disorders.
| Product Sku: | SARB0028 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 20 |
| Gene Symbols: | AFP, B2M, CA (19-9), CCL5, CEACAM5, CRP, CXCL8, DKK1, FTH1, GDF15|HGF, ICAM1, KLK3, LGALS3, MMP2, MUC16, SERPINE1, SPP1, TIMP1, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Alpha-fetoprotein
| Beta-2 Microglobulin
| CA125
| CA19-9
| CEA
|
CRP (C-Reactive Protein)
| DKK-1
| Ferritin
| Galectin-3
| GDF-15
|
HGF
| ICAM-1 (CD54)
| IL-8 (CXCL8)
| MMP-2
| Osteopontin (SPP1)
|
PAI-1
| PSA-free
| RANTES (CCL5)
| TIMP-1
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cancer Discovery Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
