Description
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Human Cytokine Array (42 targets) (SARB0037)
The Human Cytokine Array - 42 Targets (SARB0037) is a powerful tool for researchers studying cytokine expression in human samples. This array allows for the simultaneous detection of 42 different cytokines, providing a comprehensive overview of the immune response in various biological samples.With targets including pro-inflammatory cytokines, chemokines, growth factors, and other immune-related molecules, this array is perfect for studies in immunology, inflammation, cancer research, and more. The high sensitivity and specificity of this array ensure reliable and accurate results, allowing researchers to uncover novel insights into cytokine regulation and function.
Whether studying disease pathology, drug development, or biomarker discovery, the Human Cytokine Array - 42 Targets (SARB0037) is a versatile tool that can accelerate research and lead to new discoveries in the field of immunology. Enhance your studies with this comprehensive array and unlock the potential of cytokine analysis in your research.
| Product Sku: | SARB0037 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 42 |
| Gene Symbols: | ANG, CCL1, CCL15, CCL17, CCL2, CCL22, CCL5, CCL7, CCL8, CSF1|CSF2, CSF3, CXCL1, CXCL12, CXCL2, CXCL3, CXCL5, CXCL8, CXCL9, EGF, IFNG, IGF1, IL10, IL12A, IL13, IL15, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, KITLG, LEP, LTA, OSM, PDGFB, TGFB1, THPO, TNF, TPO, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
ENA-78 (CXCL5)
| GCSF
| GM-CSF
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
|
I-309 (TCA-3/CCL1)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-7
| IL-8 (CXCL8)
|
IL-10
| IL-12 p40/p70
| IL-13
| IL-15
| IFN-gamma
|
MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| M-CSF
| MDC (CCL22)
|
MIG (CXCL9)
| MIP-1 delta (CCL15)
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TNF alpha
| TNF beta (TNFSF1B)
| EGF
|
IGF-1
| Angiogenin
| Oncostatin M
| Thrombopoietin (TPO)
| VEGF-A
|
PDGF-BB
| Leptin
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
