The Human ET-1 (Endothelin-1) ELISA Kit is specifically designed for the precise quantitative measurement of Endothelin-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for various research applications.Endothelin-1 is a vital peptide involved in vasoconstriction and regulation of blood pressure, making it a key player in cardiovascular diseases and hypertension. Its dysregulation has been linked to conditions such as pulmonary hypertension, heart failure, and kidney diseases, highlighting its importance as a potential biomarker for disease diagnosis and monitoring.Overall, the Human ET-1 ELISA Kit provides researchers with a valuable tool for investigating the role of Endothelin-1 in various physiological and pathological processes, ultimately contributing to the development of innovative therapeutic strategies.
Product Name:
Human ET-1 (Endothelin 1) ELISA Kit
SKU:
HUES01351
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 pg/mL
Detection range:
1.25-80 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
