Human GPBB Recombinant Protein (RPPB1729)
- SKU:
- RPPB1729
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P11216
- Research Area:
- Enzymes
Description
| Product Name: | Human GPBB Recombinant Protein |
| Product Code: | RPPB1729 |
| Size: | 20µg |
| Species: | Human |
| Target: | GPBB |
| Synonyms: | Glycogen phosphorylase brain form, EC 2.4.1.1, GPBB, MGC9213, PYGB. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colourless liquid formualtion. |
| Formulation: | Each mg of protein contains 50% glycerol. |
| Stability: | GPBB although stable at 10°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Glycogen phosphorylase is one of the phosphorylaseenzymes(EC2.4.1.1). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule, and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism, it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase.Glycogen phosphorylase can only act on linearchainsof glycogen(a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch(which are exceedingly common in glycogen). In these situations, a debranching enzymeis necessary, which will straighten out the chain in that area. Additionally, an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done, glycogen phosphorylase can continue.An insulinstimulated enzyme known as phosphoprotein phosphatase(PP-1) inactivates glycogen phosphorylase to prevent glycogen break up.GPBB - a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. GPBB can also be a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.
Glycogen Phosphorylase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain. The Human GPBB mature chain: 2 - 843 aa; that is a total of 842 aa having a molecular mass of 96695.96 Dalton. The theoretical pI is 6.40.The GPBB is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family. |
| UniProt Protein Details: | Protein type:Phosphorylase; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Transferase; EC 2.4.1.1 Chromosomal Location of Human Ortholog: 20p11.21 Cellular Component: cytoplasm; membrane Molecular Function:glycogen phosphorylase activity; protein binding; pyridoxal phosphate binding Biological Process: glycogen catabolic process |
| NCBI Summary: | The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11216 |
| NCBI GenInfo Identifier: | 20178317 |
| NCBI Gene ID: | 5834 |
| NCBI Accession: | P11216.5 |
| UniProt Secondary Accession: | P11216,Q96AK1, Q9NPX8, |
| UniProt Related Accession: | P11216 |
| Molecular Weight: | 96,696 Da |
| NCBI Full Name: | Glycogen phosphorylase, brain form |
| NCBI Synonym Full Names: | phosphorylase, glycogen; brain |
| NCBI Official Symbol: | PYGB�� |
| NCBI Official Synonym Symbols: | GPBB�� |
| NCBI Protein Information: | glycogen phosphorylase, brain form |
| UniProt Protein Name: | Glycogen phosphorylase, brain form |
| Protein Family: | Glycogen phosphorylase |
| UniProt Gene Name: | PYGB�� |
| UniProt Entry Name: | PYGB_HUMAN |
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