The Human IL-2R Alpha ELISA Kit is specifically designed for the precise quantification of IL-2R Alpha levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and consistent results for various research applications.IL-2R Alpha, also known as CD25, is a protein involved in the immune response, specifically in the activation and proliferation of T cells.
Dysregulation of IL-2R Alpha has been linked to various autoimmune diseases, inflammatory conditions, and cancer, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.With its reliable performance and ease of use, the Human IL-2R Alpha ELISA Kit is an essential tool for researchers and clinicians interested in investigating the role of IL-2R Alpha in health and disease.
Product Name:
Human IL-2R alpha ELISA Kit
SKU:
HUES02134
Target:
Human IL-2R alpha
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Rα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2Rα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2Rα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-2Rα. You can calculate the concentration of Human IL-2Rα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
98-111
88-101
Average (%)
97
104
94
1:4
Range (%)
91-107
81-94
85-97
Average (%)
99
86
90
1:8
Range (%)
91-108
82-95
87-101
Average (%)
98
87
93
1:16
Range (%)
88-102
83-95
84-98
Average (%)
94
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
295.49
623.35
2430.39
307.44
595.04
2280.42
Standard deviation
16.75
30.05
122.25
19.83
23.92
85.06
C V (%)
5.67
4.82
5.03
6.45
4.02
3.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-2Rα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-2Rα in samples. No significant cross-reactivity or interference between Human IL-2Rα and analogues was observed.
